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Research Articles Issue 2 · 2022 · pp. 307–321 · Issue page

ASSESSMENT OF CYTOTOXIC AND APOPTOTIC EFFECTS OF EXTRACTS OF TANACETUM ARGENTEUM SUBSP. ARGENTEUM IN LIVER AND BREAST CANCER CELL LINES

DA
Damla KAYALI1
BE
Betul Eda KORHAN2
SU
Sude CAVDAROGLU3
AL
Ali SEN4
RA
Ranan Gulhan AKTAS5
1 Cancer and Stem Cell Research Center, Maltepe University, Istanbul, Turkey ; Department of Histology and Embryology, Faculty of Medicine, Atlas University, Istanbul, Turkey
2 Faculty of Medicine, Maltepe University, Istanbul, Turkey
3 Faculty of Medicine, Maltepe University, Istanbul, Turkey.
4 Department of Pharmacognosy, Faculty of Pharmacy, Marmara University, Istanbul ,Turkey
5 Department of Histology and Embryology, Faculty of Medicine, Maltepe University, Istanbul, Turkey.
Corresponding author: [email protected]
DOI https://doi.org/10.38173/RST.2022.24.2.23:307-321
Received 15 August 2022
Revised 27 August 2022
Accepted 19 October 2022
Available Online 15 November 2022
Abstract
THE STUDY AI MED TO INVESTIGATE THE ANTICANCER EFFECTS OF HEXANE, CHLOROFORM, METHANOL, AND AQUEOUS METHANOL EXTRACTS OF TANACETUM ARGENTEUM SUBSP. ARGENTEUM (TA) AGAINST BREAST CANCER CELL LINE (MCF -7) AND HUMAN LIVER CANCER CELL LINE (HEPG2) IN VITRO. ANTICANCER, ANTI-INFLAMMATORY, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF TANACETUM SPECIES HAVE BEEN ATTRACTED SCIENTISTS FOR YEARS. CELL VIABILITY WAS DETECTED BY MTT ASSAY AT DIFFERENT CONCENTRATIONS (100, 50, 25, 10, 2.5, AND 1 μG/ML). DRIED AERIAL PARTS OF TA WERE EXTRACTED WITH METHANOL (TAM) AND PARTITIONED BETWEEN WATER AND SUBSEQUENTLY N -HEXANE (TAH) AND CHLOROFORM (TAC).TAC WITH AN IC50VALUE OF 13,66 ΜG/ML EXTRACT ON HEPG2 CELLS AND TAM WITH AN IC50 VALUE OF 48,4 ΜG/ML EXTRACT ON MCF -7 CELLS SHOWED THE MOST POT ENT ANTIPROLIFERATIVE ACTIVITY IN A CONCENTRATION -DEPENDENT MANNER AT 24H TIME. THEREFORE, THESE GROUPS ARE USED FOR FURTHER IMMUNOHISTOCHEMICAL ANALYSES. AFP EXPRESSION HAS DETECTED A MEANINGFUL (P<0,05) DECREASE BETWEEN CONTROL GROUPS AND HEPG2 TAC IC50. P53 EXPRESSION DEMONSTRATE SIGNIFICANT (P<0,05) INCREASE IN HEPG2 TAC IC50 GROUP COMPARED TO THE CONTROL. THUS, THE RESULTS OF OUR STUDY SUGGEST THAT TAH AND TAC EXTRACTS ON HEPG2 CELLS ALSO TAH AND TAM EXTRACTS ON MCF -7 CELLS MIGHT HAVE HIGH ANTICANCER EFFECTS.
Keywords
TANACETUM ARGENTEUM HEPATOCELLULAR CARCINOMA BREAST
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