THE EFFECTS OF TEMPERATURE AND CARBONDIOXIDE CHANGES RELATED TO TYPE I COLLAGEN PRESENCE ON HEPATOCELLULAR CARCINOMA CELLS
Corresponding author:
[email protected]
Received
09 July 2020
Revised
-
Accepted
14 October 2020
Available Online
15 November 2020
Abstract
THE EARLY PROGNOSIS OF HEPATOCELLULAR CARCINOMA (HCC) IS EXTREMELY DIFFICULT AND TREATMENT OPTIONS ARE STILL VERY LIMITED. THE STUDY AIMS TO EXAMINE THE EFFECTS OF TEMPERATURE AND CA RBONDIOXIDE CHANGES RELATED TO THE PRESENCE OF TYPE I COLLAGEN, ONE OF THE MOS T COMMON COMPONE NTS OF THE EXTRACELLULAR MATRIX. TWO- DIMENSIONAL HEPG2 CELL CULTURES WERE MAINTAINED IN BOTH CLASSICAL CULTURE CONDITIONS AND TYPE I COLLAGEN -COATED CULTURE VESSE LS AT +4C°, +24C°, AND +37C°. A COMPARATIVE EXAMINATION OF PHENOTYPIC CHANGES WAS PERFORMED AF TER 24, 48, 72, 96, AND 120 HOURS. THE CELLS THAT ARE IN THE INCUBATORS AT 37C° WITH 5% CO 2 DEMONSTRATED NORMAL GROWTH PATTERN . ALBUMIN EXPRESSI ON WAS HIGHER IN T HE CELLS ON TYPE I COLLAGEN COATED SURFACES. CARBONDIOXIDE DEPLETION CAUSED MO RPHOLOGICAL CHANGES AND A GRADUAL DECREASE IN VIABILITY. THE CANCER CELLS ON TYPE I COLLAGEN - COATED SURFACES PRESENTED SIGNIFICANT PHENOTYPIC CHANGES IN ALL GRO UPS. THE RESULTS O F THE STUDY SUGGEST THAT THE CELLS ON TYPE I COLLAGE N-COATED SURFACES WERE MOR E RESI STANT TO T HE TEMPERATURE AND THE CARBONDI OXIDE CHANGES IN THE MICROENVIRONMENT. FURTHER STUDI ES RELATED TO THE MECHANISM(S) THAT LEADS TO THIS REZISTANCE IN CANCER CELLS MI GHT BE I NSIGHTFUL FOR T HE DEVELOPMENT OF NEW THERAPEUTICS.
Keywords
CANCER
EXTRACELLULAR MATRIX
HEPG2
COLLAGEN TYPE I
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